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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Verification of transformation of rhododendron leaf segments
 SKOTNICOVÁ, Petra
The selection procedure on kanamycin for rhododendron leaf segments of two species after transformation by Agrobacterium tumefaciens was examined in this thesis. GUS activity and presence of gene gus by polymerase chain reaction (PCR) was determined with plants regenerated on medium with concentration of kanamycin 100mg/l.
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A comparison of efficacy of direct and indirect methods of genetic transformation of potato (Solanum tuberosum L.)
 PŘIBYLOVÁ, Marie
Potato is one of the main targets for genetic improvement by gene transfer. The aim of this study was to compare the efficacy of genetic transformation of potato, cultivar Bintje, using two methods: Agrobacterium tumefaciens mediated transformation and microprojectile bombardment. The same plasmid p35SGUSint, which cosists of 35S CaMV promoter, gus and nptII genes, was used for both transformations of internodal potato explants. Kamamycin selection, transient and stable expressions of {$\beta$}-glucuronidase and PCR amplification of gus and nptII transgenes were used for transgenic plant selection, identification and analysis.
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